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KMID : 0378019750180081043
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New Medical Journal
1975 Volume.18 No. 8 p.1043 ~ p.1048
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Effects of Bilirubin and Hemoglobin on the Enzymatic Determination of Total Serum Cholesterol
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Abstract
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Effects of bilirubin and hemoglobin on the enzymatic determination of total serum cholesterol were evaluated and compared to those on the Liebermann-Buchard reaction method. Pooled serum was used for the test samples adding a sufficient amount of hemoglobin and bilirubin as shown in the Table 1 and 2. The data from the analysis of these samples show following results.
1. Serum hemoglobin concentrations of less than 600 mg/100 ml showed negligible effect on the results of cholesterol determined by the enzymatic method, while the results determined by the Liebermann-Buchard reaction method showed higher cholesterol levels in proportion of 2.5 mg/100 ml to 100 mg/100 ml of added hemoglobin.
2. Serum bilirubin concentrations have no effect on the results for cholesterol determined by the enzymatic method however the results determined by the Liebermann-Buchard reaction method showed an increased cholesterol level in proportion of 10 mg/100 ml to 2.5 mg/100 ml of added bilirubin.
3. When the bilirulin concentration is above 4.5 mg/100 ml, the results of cholesterol obtained Jby the Liebermann-Buchard reaction method can be corrected using the following formula to avoid. the error induced by bilirubin.
C=LC- (3B+5)
C: Corrected cholesterol value, LC: Cholesterol value obtained by the Liebermann-Buchard reaction method, B: Bilirublin concentration of sample(mg/100 ml)
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